Abstract In the present study, a novel Schiff base of salicylaldehyde was synthesized. Salicylaldehyde-based azomethine was synthesized by the condensation reaction of heteroatom-containing polyamine and aldehydes in acetonitrile in a non-catalyst medium and characterised by 1H-NMR, 13C-NMR analysis. The major advantage of the reaction is that it eliminates the requirement for a particular purification process, such column chromatography, for the obtained chemicals. Graphene oxide nanolayers, the second component, have been produced using a modified Hummer technique. The oxidising agent concentration (H2SO4+KMnO4) was increased, allowing for the receipt of purer nanolayers, which is what led to the method's change. Through the use of SEM and XRD techniques to examine the structure and morphology of nanolayers, it was shown that the thickness of these layers is 1 nm. Azomethine was then used to modify graphene oxide. Subsequently, graphene oxide was modified with azomethine. The compound and the ensemble were screened for antibacterial (S. aureus, E. coli, A. baumannii, P. aeruginosa, K. pneumonia) activities. The minimum inhibitory concentrations of the compounds were also ascertained by the two-fold micro-dilution method. A study revealed that azomethine's antibacterial activity might be enhanced through the addition of graphene oxide nanolayers into the molecule itself.