As known green nanotechnology is the possibility of synthesizing metal nanoparticles using extracts of plants and is being actively developed as an alternative, efficient, cheap and environmentally safe method of producing nanoparticles with specified properties. This work focuses on the synthesis of Ag and CuO nanoparticles in the presence of bioactive substances found in the leaves, roots, peels of medicinal plants and fruits, such as curcuma and pomegranate. The main research methods were UV-vis. spectroscopy and disc diffusion (Kirby-Bauer) method. It has been shown the results of the antibacterial activity of prepared nanoparticles using samples of cotton fabric modified with them.