The paper presents the results of the analysis of electrophysiological parameters of isolated cells of two species of algae taken from separate natural water bodies of Azerbaijan. Chara gymnophylla cells were collected from Guzgugol lake, which is part of the lake system of the Goygol lake Nature Reserve. Chara fragilis cells were collected from a small water body in the Katib Bulagy fount of Tovuz district. Isolated cells from both plants were subjected to detailed electrophysiological analysis and proved to be unique for the analysis of ion exchange patterns and electrogenic transport between the environment and the cell, using glass microelectrodes. A comparison of the electrophysiological properties of the noted species of charophytes is carried out for the first time.