Wheat, a globally critical cereal crop, is profoundly affected by drought stress, necessitating advanced strategies to enhance its resilience. This study evaluates the expression level of the TaWXPL1D transcription factor (TF) gene, which plays a pivotal role in cuticle biosynthesis, across two bread wheat genotypes, Aran and Gyzyl bughda, under control and drought conditions. Gene expression was analyzed five days post-initiation of water deficit, a timepoint marked by the manifestation of visible stress symptoms. Quantitative real-time PCR (qRT-PCR) was employed to quantify transcript levels, utilizing elongation factor 1 alpha (Elf1-α) as a reference gene for normalization. Under optimal conditions, TaWXPL1D expression exhibited no significant genotypic variation. However, drought stress induced significant transcriptional alterations, with a pronounced downregulation observed in the drought-sensitive genotype Aran. These results underscore genotype-specific regulatory mechanisms influencing cuticle development under drought stress and provide a foundation for targeted breeding approaches to improve drought tolerance in wheat.