For this molecular genetic research, helminthological samples were collected from the lungs of 17 samples of Pelophylax terentievi (Mezhzherin, 1992) distributed in the Kashkadarya and Surkhondarya regions in the spring and summer of 2024. The difference between the nucleotides of the R. engelbrechti_uz (PQ219671) sample belonging to the Rhabdias genus collected from the southern regions of our republic and the R. engelbrechti (MG428406) sample obtained from the NCBI database was 0.4%. The nucleotide sequence obtained from the molecular-genetic study of Rhabdias engelbrechti belonging to the Rhabdias genus was placed in the National Center for Biotechnology Information. To the best of our knowledge, this species was recorded for the first time in the territory of the Republic of Uzbekistan.